PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.

31 – 45 of 135 results

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10F' Chemically Competent E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid. Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10F´
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot
Cell Type	Chemically Competent

Invitrogen™ TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1^R E. coli

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Pricing & Availability
Specifications

Promoter	T7, SP6
Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	DH5α
Vector	TOPO-TA Cloning Vectors
Product Line	One Shot

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ MAX Efficiency DH5α-T1^R E. coli

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Pricing & Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	DH5α
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Electrocomp™ E. coli

Offers the fastest and easiest method for high-efficiency cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

Pricing & Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
Vector	Blunt DNA Cloning Vectors
Product Line	One Shot

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ TOP10F' Chemically Competent E. coli

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Pricing & Availability
Specifications

Promoter	T7, SP6
Includes	Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Content And Storage	This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	TOP10F ́
For Use With (Application)	PCR Cloning
Vector	pCRII
Product Line	One Shot
Cell Type	Chemically Competent E. coli

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

Pricing & Availability

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells

Enables rapid cloning of one or more genes into virtually any protein expression system

Pricing & Availability

Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF' Chemically Competent E. coli

Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector

Pricing & Availability
Specifications

Promoter	T7, SP6
Includes	Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Content And Storage	This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	INVαF ́
For Use With (Application)	PCR Cloning
Vector	pCRII
Product Line	One Shot
Cell Type	Chemically Competent E. coli

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1^R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

Pricing & Availability

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1^R Chemically Competent E. coli

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

Pricing & Availability

Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

Pricing & Availability

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10 E. coli (Supply Center Packaging)

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Pricing & Availability

Invitrogen™ TOPO™ TA Kit, TOP10F' (Supply Center Packaging)

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

Pricing & Availability

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process

Pricing & Availability

Invitrogen™ Bac-to-Bac™ N-His TOPO™ Cloning Kit

Bac-to-Bac™ N-His TOPO™ Cloning Kit

Pricing & Availability

PCR Cloning Kits

Filtered Search Results

Narrow Results